6x-His Tag Monoclonal Antibody (3D5), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen R93025

Product Code. 10193852

Description

NOTE: R930-25 was previously sold under product #46-0693. When re-ordering, please use #R930-25. This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells. This product contains enough material for 25 Western blots. It is functionally tested using 500 ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. A signal can be detected when tested against 100 ng of recombinant Positope™ protein. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM). This product has also been used successfully to immunoprecipitate fusion proteins that contain the 6x His tag. Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

6x-His Tag Monoclonal antibody specifically detects 6x-His Tag in Tag samples. It is validated for ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot.

Specifications

• Antigen: 6x-His Tag
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
• Immunogen: 6x His synthetic peptide
• Quantity: 50 μL
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: 3D5
• Formulation: PBS with 0.01% sodium azide
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Tag