eBioscience™ Human IL-17E (IL-25), Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8736-62

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15598826

Quantity Price
1 £ 66.65 / 5µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

Recombinant Yes
Name IL-17E (IL-25)
Conjugate Unlabeled
Regulatory Status RUO
Quantity 5μg
Cross Reactivity Human
Biological Activity This protein (10-100 ng) can to induce IL-8 secretion from normal human peripheral blood cells.
Purity >98%
Structural Form E. coli (amino acids Met17-Gly; Accession # AAI04930.1)
Formulation Lyophilized from 10mM acetic acid
Purification Method SDS-PAGE and HPLC
For Use With (Application) Functional Assays
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Product Type Recombinant Protein, Carrier-Free
Molecular Weight 33.8kDa disulfide-linked homodimer of two 145 aa polypeptide chains
Gene Symbol Interleukin-17E, Interleukin-25
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Source E. coli

IL-17E, or IL-25, was first identified as an IL-17 family member on the basis of sequence homology. It was originally believed to be a Th2 cytokine based on the detection of mRNA transcripts in polarized Th2 cells, but expression was later observed in epithelial cells, eosinophils, and mast cells. Studies have confirmed the role of IL-17E as a potent inducer of type 2 immune responses. IL-17E signaling induces the differentiation of CD4+ T cells towards the Th2 lineage and upregulates expression of Th2 cytokines such as IL-4, IL-5, and IL-13. Administration of IL-17E also promotes symptoms of allergic asthma, such as eosinophil infiltration, airway hyperreactivity, and high circulating levels of IgE. IL-17E binds to IL-17BR, a receptor shared with IL-17B, and activates NF-kappa B pathways.