eBioscience™ Human CD154 (CD40 Ligand), sCD154 (sCD40 Ligand), Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 34-8902-81

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15558836

Quantity Price
1 £ 152.25 / 50µg
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Regulatory Status RUO
Cross Reactivity Human
Conjugate Unlabeled
Molecular Weight 16.3kDa
Biological Activity The ED50 of this protein, as measured by stimulation of IL-8 production by normal human peripheral blood cells, is less than or equal to 5-10ng/mL.
For Use With (Application) Functional Assays
Purification Method SDS-PAGE and HPLC
Quantity 50μg
Gene Symbol CD40L, Tnfsf5, TRAP
Name CD154 (CD40 Ligand), sCD154 (sCD40 Ligand)
Purity >98%
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Product Type Recombinant Protein, Carrier-Free
Recombinant Yes
Source E. coli
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Structural Form E. coli (amino acids Gln114-Leu261; Accession # NP_000065.1)

CD40 ligand, (CD40L, also known as CD154, TRAP or gp39) is a membrane glycoprotein expressed on activated CD4+ T-cells, NK cells, mast cells, basophils and eosinophils. The CD40-CD40L interaction stimulates B cell immune response which includes cell surface antigen expression, cell cycle activation, Ig isotype switching, Ig secretion and memory generation. The CD40-CD40L interaction also plays important roles in monocyte and dendritic cell activation, T-cell co-stimulation and cytokine production. It has been reported that the CD40-CD40L interaction is involved in the pathogenesis of amyloid pathology in Alzheimer disease.