missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ eBioscience™ Annexin V-Biotin Apoptosis Detection Kit

Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

Brand:  Invitrogen™ BMS500BT-100

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15551947

  • £165.02 / 100 tests


Call for latest availability
+44 (0)1509 555 500
Add to basket

Disclaimers:

For Research Use Only


Includes: Annexin V-Biotin protein
Binding Buffer (4x)
Propidium Iodide (20 ug/mL)

Description

Description

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V.

In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD.

Specifications

Specifications

100 tests
Flow Cytometric Analysis
35.8 kd
Biotin
> 98%
Lipocortin V, Endonexin II, Calphobindin I
Videos