Angiogenin, clone: 26-2F, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9762-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 26-2F recognizes human angiogenin. Angiogenin is a secreted ribonuclease (RNASE)-family member and is important for the formation of new blood vessels as well as the stimulation of cell proliferation and survival. Cellular stress signals direct angiogenin to stress granules where angiogenin functions by hydrolyzing cellular tRNA resulting in modulation of protein translation and promotion of cell survival. Cytoplasmic angiogenin can bind to actin, forming complexes that activate protease cascades which can degrade laminin and fibronectin allowing endothelial cells to penetrate the basement membrane and migrate. In cancer cells, growth signals direct angiogenin translocation to the nucleus where binding of DNA promotor regions stimulates rRNA transcription resulting in a downstream increase in protein synthesis driving cell growth and survival. Angiogenin is upregulated in multiple cancers including breast, prostate, cervical, colorectal, lymphoma, and melanoma. During development and in adulthood, angiogenin is highly expressed in the spinal cord and contributes to motor neuron survival. Angiogenin is important for neuronal survival and is depleted in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and Parkinson's disease. This 26-2F antibody is reported to have both in vivo and in vitro neutralizing activity to angiogenin.Applications Reported: This 26-2F antibody has been reported for use in cytokine neutralization, immunoprecipitation, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, ELISA, and immunocytochemistry.Applications Tested: This 26-2F antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|