Amphiregulin Monoclonal Antibody (AREG559), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15963244	Affymetrix eBioscience 25-5370-42	100 Tests
15953244	Affymetrix eBioscience 25-5370-41	25 Tests

Description

Description: The AREG858 monoclonal antibody reacts with human Amphiregulin (AR). AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage. Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes. In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation. Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils. Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AREG559 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Amphiregulin Monoclonal antibody specifically detects Amphiregulin in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Amphiregulin
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P15514
• Gene Symbols: AREG
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: AREG

• Clone: AREG559
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 374
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody