Invitrogen™ Ambion™ TaqMan™ Gene Expression Cells-to-CT™ Kit

Perform expression analysis directly from cultured cells without RNA purification

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1729

400 RXN GENE EXPRESSION CELLS-TO-CT KIT 400REACTIOns Store at -20 C

UNSPSC: 41106302

Code: 50

Additional Details:
Additional Details: Weight: 0.27000kg



Product Code. 10664844

Quantity Price
1 £ 3125.0 / 400 reactions
EU Stock 12
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Detection Method Primer-Probe Detection
Dye Type ROX (Pre-mixed)
For Use With (Application) Drug Discovery and Development, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, PCR-Based Drug Discovery Assays, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples, Target and Lead Identification and Validation, siRNA
Includes Stop Solution: 2 x 1mL tubes; DNase I: 220μL tube; 20X RT Enzyme Mix: 1.1mL tube; Lysis Solution: 22mL bottle; 2X RT Buffer: 22mL bottle; TaqMan Gene Expression Master Mix: 4 x 5mL bottles
No. of Tests 400.0
Storage Requirements Store Stop Solution, DNase I, and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X RT Buffer, and TaqMan Gene Expression Master Mix at 4°C.

Kkit saves time and offers simple workflow suitable for few samples or can be easily incorporated into automated, high-throughput applications.

  • Complete: optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TAqMan Gene Expression Master Mix
  • Fast: 7-minute sample prep, including DNase treatment, at room temperature
  • Easy: lyse samples in a tube or directly in culture plates
  • Robust: perform gene expression analysis on 10-100,000 cells per sample; results equivalent to those from purified RNA
  • Efficient: contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples
  • Fewer resources used, less hazardous, less waste

Drug Discovery and Development, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, PCR-Based Drug Discovery Assays, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples, Target and Lead Identification and Validation