Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL

Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues

Brand:  Invitrogen™ AM2294

Product Code. 10545195

Description

RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays.

• Supplied in one tube of 10,000U (100U/μL)
• Endonuclease-free, exonuclease-free, protease-free
• Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
• Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min. at 37°C

DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting

Specifications

• Concentration: 100 U/μL
• Shipping Condition: Dry Ice
• Quantity: 10,000 U
• Product Line: Ambion™

• Content And Storage: -20°C
• Enzyme: RNase
• Product Type: RNase I

For Research Use Only. Not for use in diagnostic procedures.