Store β-Actin Primer Pair and β-Actin Competimers at -20°C. Store Control Template RNA below -70°C. Store Nuclease-free water at any temperature.
The Ambion QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimers of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimers to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed“housekeeping”gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp