Store E-PAP, 5X E-PAP Buffer, ATP Solution, MnCl2 and Control DNA Template at -20°C. Store Nuclease-free Water at any temperature.
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions. The Poly(A) Tailing Kit uses E. coli Poly(A) Polymerase I (E-PAP) to polyadenylate the 3'-termini of in vitro transcribed RNA. Polyadenylation plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. In the Poly(A) Tailing Kit, the E-PAP reaction has been optimized so that mRNAs are efficiently tailed with at least 150 adenines. The additional adenine residues confer stability to the mRNA and may increase translational efficiency of in vitro synthesized capped RNA in microinjection and transfection experiments.
Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
Enhances translational efficiency in vivo
Reaction can be adjusted to control for tail length
Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit
Accessory Products: The Poly(A) Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE High Yield Capped RNA Transcription Kit. mMESSAGE mMACHINE Kits are available with T7 (Cat. No. AM1334), T3 (Cat. No. AM1338), or SP6 (Cat. No.AM1330) polymerases.
Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, In Vitro Translation, Protein Expression, Proteins, Expression, Isolation and Analysis