Invitrogen™ Ambion™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1348M

25 RXN MMESSAGE MMACHINE T3 KIT WITH MANUAL 25REAC

UNSPSC: 41105804

Code: 50

Additional Details:
Additional Details: Weight: 0.07000kg



Product Code. 10187294

Quantity Price
1 £ 390.0 / Each
Estimated Shipment date
from Supplier 07-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T3 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Product Type Kit with manual
Quantity 25 reactions

Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

  • High yields of capped RNA—up to 39μg of RNA per reaction
  • Extra convenience—cap analog-nucleotide mix provided
  • Simplified reaction format
  • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA
  • 10–50 times yield obtained with conventional in vitro transcription reactions
  • Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%)
  • Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
  • Optimized for use with polymerases included in kit
  • Contains 57U of cap analog