Invitrogen™ Ambion™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Overview
Brand: Invitrogen™

Manufacturer Part Number: AM1700

RLM-RACE KIT 5/10/100 RXNSsufficient reagents for 100 rxns

UNSPSC: 41106300

Code: 50

Additional Details:
Additional Details: Weight: 0.11000kg



Requires: SuperTaq Thermostable Taq DNA Polymerase sold separately. For optimal amplification of fragments ≥1 kb, use SuperTaq-Plus.

Product Code. 10554445

Quantity Price
1 £ 534.0 / Each
EU Stock 23
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Includes Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Quantity 1 kit

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

  • From RNA to PCR product in less than a day
  • Yields single, specific product from rare transcripts
  • Selects 5' and/or 3' ends of true messages
  • Efficient—
  • Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
  • Final product size: 7kb or less
  • Optimal reaction temperature: 42°C
  • Sample: RNA

  • Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
  • Improvement to basic RACE protocol
  • Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction