Alpha-Smooth Muscle Actin, clone: 1A4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9760-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin. Alpha-smooth muscle actin is a 42kDa protein and is a major component of the cytoskeletal structural network. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in the parenchyma and stroma of various tissues. Alpha-smooth muscle actin is useful in the identification of leiomyomas, leiomycosarcomas and pleomorphic adenomas and is used as a prognostic marker for basal cell carcinoma.Applications Reported: This 1A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. (Fluorochrome conjugated 1A4 is recommended for use in intracellular flow cytometry.).Applications Tested: This 1A4 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 1 μg/mL. This 1A4 antibody has been tested by immunocytochemistry of formaldehyde or methanol-fixed and permeabilized human cells and can be used at 1 μg/mL. This 1A4 antibody has been tested by western blot analysis of reduced rat and mouse intestine and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Alpha-Smooth Muscle Actin|
|PBS with 0.09% sodium azide; pH 7.2|
|P62737, P62736, P62738|
|Actin, aortic smooth muscle, Alpha Actin 2, alpha-sma|
|Flow CytometryþImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þImmunoprecipitationþMicroscopyþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.