alpha GalCer:CD1d Complex, Functional Grade, clone: L363, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-2019-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes.Applications Reported: This L363 antibody has been reported for use in functional assays.Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylcermide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|alpha GalCer:CD1d complex|
|PBS with no preservative; pH 7.2|
|alpha GalCer-CD1d complex|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.