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TCR alpha/beta Monoclonal Antibody (IP26), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-9986-42
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The IP26 monoclonal antibody reacts with the alpha beta chain of human TCR.The alpha beta TCR is expressed by the majority of peripheral T cells. Applications Reported: This IP26 antibody has been reported for use in flow cytometric analysis. Applications Tested: This IP26 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
TCR alpha/beta Monoclonal antibody specifically detects TCR alpha/beta in Human samples. It is validated for Flow Cytometry.Specifications
TCR alpha/beta | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P01848, P04435 | |
TRA, TRAC, TRB, TRBV7-9 | |
Affinity chromatography | |
RUO | |
28589, 28755, 6955, 6957 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
IP26 | |
PerCP-eFluor 710 | |
TRB | |
FLJ22602; IMD7; LOC290071; MGC117436; MGC22624; MGC23964; MGC71411; RATTCB; RATTCBC1; RGD1359684; similar to RIKEN cDNA A430107P09 gene; T cell receptor beta locus; T3/TCR complex; TCB; TCBC1; t-cell antigen receptor; T-cell receptor alpha constant; T-cell receptor beta chain; T-cell receptor V alpha; tcr alpha; TCR alpha/ beta; TCR beta; TCRA; Tcrb; TRA; Tra29; TRAC; TRB; TRB@; TRCA | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.