alpha beta TCR Mouse anti-Human, PE, Clone: WT31, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-9955-42

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15547286

Quantity Price
1 £ 133.91 / 100 tests
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Quantity 100 tests
Conjugate PE
Isotype IgG1
Regulatory Status RUO
Clone WT31
Host Species Mouse
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Species Reactivity Human
Antigen alpha beta TCR
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Concentration 5μL (0.06μg)/test
Applications Flow Cytometry

This WT31 monoclonal antibody recognizes the human alpha beta T cell receptor (TCR) complexed with the CD3 epsilon chain. Composed of a 44kDa alpha and a 37kDa beta chain, TCR specificity is typically determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events lead to T cell activation, resulting in cellular signaling and cytokine production.

The WT31 monoclonal antibody has been reported to induce proliferation of resting T cells, as well as inhibit cytotoxic T cell activity. In addition, some antibodies against CD3 partially inhibit alpha beta TCR antibody staining using WT31; clone SK7 (anti-human CD3) is recommended for use in two-color experiments.