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APC, clone: eBioAPC-6A2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-4170-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15237357

  • £49.41 / 50µg


Estimated Shipment
02-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested.Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioAPC-6A2 antibody has been tested flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to by μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Allophycocyanin
eBioAPC-6A2
Unconjugated
Purified
Murine
50μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Anti-APC
IgG1
RUO
Primary
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