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AIRE, eFluor 660, clone: 5H12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5934-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15519996

  • £88.18 / 25Āµg


EU Stock 2
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Description

Description

Description: This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE). This 58-kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). However, expression of AIRE protein in the periphery remains unclear despite the detection of AIRE mRNA in the lymph node, lymphoid stromal cells, monocytes, and dendritic cells. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation.The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.Applications Reported: This 5H12 antibody has been reported for use in intracellular flow cytometric analysis and immunohistochemical staining of frozen tissue sections.Applications Tested: This 5H12 antibody has been tested by immunohistochemistry on frozen 5-week-old mouse thymus and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

AIRE
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9Z0E3
AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), autoimmune regulator, AIRE1, OTTHUMP00000109529, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾Immunohistochemistry (Frozen)
5H12
eFluor 660
Aire
Conjugated
Aire
IgG2c, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
11634
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