AIRE, clone: TM-724, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9534-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE). This 58kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA in the periphery has been observed in the lymph node, fetal liver, and intestines. Furthermore expression has been observed in dendritic cells, epithelial cells and PBMCs. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. As a result of alternative splicing, three isoforms have been reported. The conditions autoimmune-polyendocrinopathy-candiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1) arise from mutations in the human AIRE gene.The TM-724 antibody is predicted to recognize all three isoforms of human AIRE (AIRE-1, AIRE-2, AIRE-3).Applications Reported: This TM-724 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This TM-724 antibody has been tested by immunohistochemistry on FFPE human thymus at less than or equal to 5 ug/mL. Low pH antigen retrieval is optimal although high pH antigen retrieval can also be used. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|