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Aiolos, eFluor 660, clone: 8B2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5789-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15331930

  • £227.10 / 100µg


Estimated Shipment date
from Supplier 26-07-2018
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Description

Description

Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 8B2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Aiolos
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O08900
AIO, AIOLOS, ZNFN1A3, ikaros family zinc finger protein 3, zinc finger protein Aiolos, zinc finger protein, subfamily 1A, 3 (Aiolos)
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
8B2
eFluor 660
Ikzf3
Conjugated
Ikzf3
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
22780
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