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AHR, Alexa Fluor 488, clone: 4MEJJ, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5925-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15550227

  • £105.77 / 25µg


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 4MEJJ monoclonal antibody recognizes mouse aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contaminants, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells.The 4MEJJ monoclonal antibody has been found to recognize the b-1, b-2 and d alleles of mouse AHR but does not crossreact with human AHR.Applications Reported: This 4MEJJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 4MEJJ antibody has been tested by intracellular staining and flow cytometric analysis of TH17 polarized mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

AHR
4MEJJ
Alexa Fluor 488
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Aryl hydrocarbon receptor
IgG2a
RUO
Primary
Murine
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