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alpha-Fetoprotein, clone: 1E8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9499-80

Code : NEW

Additional Details : Weight : 0.09500kg

Product Code. 15577596

  • £139.00 / 25µg

Estimated Shipment date
from Supplier 23-03-2020

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Description

Description

Description: This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.Applications Reported: This 1E8 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This 1E8 antibody has been tested by western blot on reduced HepG2 cell lysate and by immunohistochemistry on FFPE human liver section using high pH antigen retrieval. The antibody can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

alpha-Fetoprotein
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P02771
alpha-fetoglobulin, alpha-1-fetoprotein, AFP
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
1E8
Unconjugated
AFP
Liquid
AFP
IgG1, kappa, kappa
25μg
Store at 2-8°C.
Primary
174
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