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alpha-Fetoprotein, clone: 1E8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9499-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Alpha Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Description: This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions.Applications Reported: This 1E8 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This 1E8 antibody has been tested by western blot on reduced HepG2 cell lysate and by immunohistochemistry on FFPE human liver section using high pH antigen retrieval. The antibody can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.Specifications
alpha-Fetoprotein | |
Monoclonal | |
0.5mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P02771 | |
AFP | |
Affinity chromatography | |
RUO | |
174 | |
Store at 2-8°C. | |
Liquid |
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot | |
1E8 | |
Unconjugated | |
AFP | |
alpha-fetoglobulin, alpha-1-fetoprotein, AFP | |
Mouse | |
25 μg | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.