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Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

Brand:  Affymetrix USB 78390.1000.UN

Code : NEW

Additional Details : Weight : 0.47500kg

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Product Code. 15523687

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Specifications

Description

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis
Description & Specifications

Specifications

Dephosphorylation of DNA prior to cloning
1000 units