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Actin (muscle), clone: HHF35, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6496-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15207717

  • £117.21 / 100µg


EU Stock 2
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Description

Description

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)) and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Actin (muscle)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P68136, P68035, P62738, P68137, P68134, P68033, P62737, P68133, P68032, P62736, Q3ZC07, P68138, P62739
actin, actin alpha 1, actin alpha cardiac, actin alpha cardiac 1, actin, alpha cardiac muscle 1, actin, alpha skeletal muscle, actin, alpha, cardiac 1, actin, alpha, vascular smooth muscle, actin, aortic smooth muscle, alpha actin 1, alpha actin 2, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin cardiac, alpha-actin-1, alpha-actin-2, alpha-cardiac actin, alpha-sma, alphac-actin, cell growth-inhibiting gene 46 protein, nemaline myopathy type 3, skeletal alpha actin, smooth muscle alpha-actin
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
BovineþCanineþEquineþGuinea PigþHumanþMurineþNon-human PrimateþPorcineþRat
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þWestern Blot
HHF35
Unconjugated
ACTA2
Purified
Acta1, ACTA2, Actc1
IgG1, kappa
100μg
Store at 2-8°C.
Primary
58, 59, 70
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