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Actin (muscle), clone: HHF35, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6496-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15297707

  • £56.74 / 25µg


Estimated Shipment
02-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)) and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Actin (muscle)
HHF35
Unconjugated
Purified
IgG1
RUO
Primary
Rat
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25μg
Store at 2-8°C.
Monoclonal
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