Thermo Scientific™ Acclaim™ PepMap™ C18 Peptide Analysis HPLC Column, 5 μm, 75 μm x 50 mm

Column Format: Capillary Column; Carbon Load: 15%; Pore Size: 100 Å; Column Phase: Reversed Phase

Brand:  Thermo Scientific™ 160318

Product Code. 11332103

Description

This is the standard column for high-resolution, high-sensitivity protein identification for proteomics and biomarker discovery and verification. The Thermo Scientific™ Acclaim™ PepMap™ C18 100 HPLC Column, 5μm Particle Size is ideal for:

High-resolution peptide mapping of tryptic, natural, and synthetic peptides

LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology

High loading capacity

Acclaim PepMap columns are exceptionally suitable for the analysis of low abundant peptides often found in complex proteomics samples. The Acclaim PepMap allows for tuning throughput, sensitivity, and amount of sample available.

• C18 100Å column with 3μm particle size, 50mm length, and I.D. of 75μm
• Superior protein and peptide separations
• Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
• Outstanding separation efficiency with superior resolution and recovery
• Easy installation with nanoViper™ Fingertight Fittings
• Columns are available with Thermo Scientific™ Dionex™ nanoViper Fingertight Fittings:
• Requires no tools
• Provides virtually zero-dead-volume connections
• Withstands pressures up to a 1000 bar

About Acclaim PepMap Nano columns

Nano (75μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. Nano LC columns are often used in proteomics.

Specifications

• Column Format: Capillary Column
• Flow Rate: 200 nL/min
• Length (Metric): 50 mm
• Particle Size: 5 μm
• Pore Size: 100 Å
• USP Type: L1
• Product Line: nanoLC
• Carbon Load: 15%
• Phase: Reversed Phase

• Diameter (Metric): 75 μm
• For Use With: LC/MS, ESI-MS, MALDI-MS
• Max. Pressure: 14500 psi (1000 bar)
• pH: 2 to 8
• Surface Area: 450 m²/g
• Stationary Phase: C18
• Packing Material: Silica
• Column Type: Peptide Analysis
• Material: Fused Silica