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Abnova™ Fluorescent Dye 488-I Streptavidin
Description
Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable. Additionally, the fluorescence of Fluorescence Dye 488-I is not affected by pH between 4-10. Compared to Alexa Fluor 488, Fluorescence Dye 488-I of single isomer has much higher purity, bringing better performance consistency from batch to batch. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 488, Cy2, DyLight 488, FITC
- Molar Extinction Co-efficient: ≥70,000 M-1cm-1
- Excitation (Max): 491nm
- Emission (Max): 514nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescence Dye 488-I with Streptavidin conjugate. Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable. Additionally, the fluorescence of Fluorescence Dye 488-I is not affected by pH between 4-10. Compared to Alexa Fluor 488, “Fluorescence Dye 488-I of single isomer has much hihger purity, bringing better performance consistancy from batch to batch. |
| For Use With (Application) | Conjugation |
| Quantity | 200 μg |
| Product Type | Fluorescent Dye 488-I Streptavidin |
| Equivalence | Alexa Fluor 488, Cy2, DyLight 488, FITC |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye 488-I Streptavidin |
| Emission | 514 |
| Excitation | 491 |
For research use only (RUO).
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