3G11 Sialoganglioside Antigen Mouse anti-Mouse, Rat, eFluor 660, Clone: SM3G11, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-5443-82

100UG Anti-Mouse/Rat 3G11 Sialoganglioside Antigen eFluor 660

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15529976

Quantity Price
1 £ 260.0 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen 3G11 Sialoganglioside Antigen
Applications Flow Cytometry
Clone 3G11)
Concentration 0.2mg/mL
Conjugate eFluor 660
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Mouse
Isotype IgM
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse, Rat
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3 epsilon, suggesting close proximity of these two proteins.

Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.