Thermo Scientific 1-Step Human High-Yield In Vitro Expression Kits

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: 88891

1-STEP HUMAN HIGH-YIELD MINI IVT KIT,

UNSPSC: 41106500

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15235743

Quantity Price
1 £ 996.0 / 1 set
EU Stock 4
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Description and Specification

Specification

Description 1-Step Human High-Yield Mini IVT Kit
Formulation Multi-component kit
Kit Contents 5X Dialysis Buffer, 3mL
Quantity 10 x 100μL kit
Sufficient For 10 reactions of 100μL each

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.

Highlights:

  • High expression – up to 750μg/mL of expressed protein

  • Reproducibility – low variability between experiments

  • Fast – express high levels of protein with an overnight incubation

  • Functional – obtain more active protein than with bacterial protein expression systems

1-Step Protein Expression:

  • Streamlined reaction – requires only five components

  • Easy-to-use – transcription and translation is performed in one reaction step

  • Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids

Includes:

Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes

Requires:

30°C incubator or water bath

Recommended for:

Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production