The 1-Step Heavy Protein IVT Kit uses a unique HeLa cell lysate supplemented with heavy amino acids for in vitro translation (IVT) of proteins with 90 to 95% isotope incorporation efficiency in less than 8 hours. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample prep loss, digestion efficiency determination or as quantification standards.
Efficient – express heavy proteins with 90-95% stable isotope incorporation
Functional – uses the human translational machinery to express more biologically active proteins than other IVT systems
Flexible – express light proteins or heavy proteins containing 13C615N2 L-lysine and/or 13C615N4 L-arginine
Convenient – perform transcription and translation in a single step
High performance – greater yields compared to rabbit reticulocyte in vitro translation
Identify ideal peptides for targeted quantitation; Verify protein digestion efficiency; Control for protein sample prep variability and affinity enrichment loss