Thermo Scientific™ 1-Step Heavy Protein IVT Kit
Express stable isotope-labeled (i.e., heavy) proteins by in vitro translation (IVT) for mass spectrometry using these cell-free expression kits.
Brand: Thermo Scientific™ 88331
Code : PN
Additional Details : Weight : 0.02000kg
DescriptionThe 1-Step Heavy Protein IVT Kit uses a unique HeLa cell lysate supplemented with heavy amino acids for in vitro translation (IVT) of proteins with 90 to 95% isotope incorporation efficiency in less than 8 hours. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample prep loss, digestion efficiency determination or as quantification standards.
Efficient – express heavy proteins with 90-95% stable isotope incorporation
Functional – uses the human translational machinery to express more biologically active proteins than other IVT systems
Flexible – express light proteins or heavy proteins containing 13C615N2 L-lysine and/or 13C615N4 L-arginine
Convenient – perform transcription and translation in a single step
High performance – greater yields compared to rabbit reticulocyte in vitro translation
Identify ideal peptides for targeted quantitation; Verify protein digestion efficiency; Control for protein sample prep variability and affinity enrichment loss
|Pierce 1-Step Heavy Protein IVT Kit|
|Positive Control DNA: pCFE-GFP, 10ug, 13C6 15N2 L-Lysine, 25uL, 13C6 15N4 L-Arginine, 25uL, Accessory Proteins, 100uL, Nuclease-Free Water, 1.5mL, pT7CFE1-CGST-HA-His, 10ug, Reaction Mix, 200uL, HeLa Lysate, 500uL|
|40 reactions of 25uL each|
|40 rxn kit|