Thermo Scientific™ 1-Step Heavy Protein IVT Kit

Express stable isotope-labeled (i.e., heavy) proteins by in vitro translation (IVT) for mass spectrometry using these cell-free expression kits.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88330

1-STEP HEAVY PROTEIN IVT KIT

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12112410

Quantity Price
1 £ 237.0 / Each
Estimated Shipment date
from Supplier 21-12-2016
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Description and Specification

Specification

Description Pierce 1-Step Heavy Protein IVT Kit
Formulation Multi-component kit
Isotopic Labeling Yes
Source Material DNA
Kit Contents 13C6 15N2 L-Lysine, 25uL
Quantity 8 rxn kit
Sufficient For 8 reactions of 25uL each

The 1-Step Heavy Protein IVT Kit uses a unique HeLa cell lysate supplemented with heavy amino acids for in vitro translation (IVT) of proteins with 90 to 95% isotope incorporation efficiency in less than 8 hours. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample prep loss, digestion efficiency determination or as quantification standards.

Highlights:

  • Efficient – express heavy proteins with 90-95% stable isotope incorporation

  • Functional – uses the human translational machinery to express more biologically active proteins than other IVT systems

  • Flexible – express light proteins or heavy proteins containing 13C615N2 L-lysine and/or 13C615N4 L-arginine

  • Convenient – perform transcription and translation in a single step

  • High performance – greater yields compared to rabbit reticulocyte in vitro translation

Recommended for:

Identify ideal peptides for targeted quantitation; Verify protein digestion efficiency; Control for protein sample prep variability and affinity enrichment loss