Direct PCR Reagents and Kits

Beckman Coulter™ Agencourt AMPure XP

Trusted and recommended reagent for size selection and cleanup steps within a variety of NGS workflows. Beckman Coulter™ Agencourt AMPure XP removes unwanted contaminants from DNA in a variety of applications including PCR, NGS, cloning, and microarrays.

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Thermo Scientific™ Verso 1-Step RT-PCR Master Mixes are optimized for the detection and analysis of RNA templates in a single step.

TaqMan™ TaqMan™ Fast Virus 1-Step Master Mix

TaqMan™ Fast Virus One-Step Master Mix is designed for reliable, high-sensitivity real-time RT-PCR even in the presence of common reaction inhibitors.

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Invitrogen™ Precision gRNA Synthesis Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT1-Step Kit

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

First Strand cDNA Synthesis Kit

Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.

Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Synthesize long cDNAs up to 20 kb at elevated temperatures (up to 65°C) with this complete system for highly efficient synthesis of first strand cDNA.

SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

PCR directly from various animal tissues including mice, fish, birds and insects without DNA purification.

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix

Sartorius™ Microsart™ Research Mycoplasma Real-Time PCR Kit

For fast and reliable screening of cell culture supernatants. Microsart™ Research Mycoplasma Real-Time PCR Kit is used for direct detection of Mollicutes in cell culture, media components, and derived biologicals.

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

A complete system for efficient synthesis of first strand cDNA from RNA templates.

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Save time and to reduce the possibility of pipetting errors with the pre-mixed components in this complete system for efficient synthesis of first strand cDNA.

Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)

Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Perform PCR directly from human samples including buccal swabs, saliva, hair, and fingernails without DNA purification.

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

A single-tube, 4X RT-qPCR master mix that provides for sensitive, reproducible detection of up to four different RNA (or DNA) targets in a single multiplex reaction.

Thermo Scientific™ Verso cDNA Synthesis Kit

Perform robust transcription of RNA to create a complete cDNA pool.

Thermo Scientific™ ROX Solution, 50 µM

ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX.

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase

Complete system for efficient synthesis of first strand cDNA.

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Efficient PCR directly from different plant materials across species.

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal

Incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments.

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