Restriction Enzymes
Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ ATP Solution (100 mM)
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ MunI (MfeI) (10 U/μL)
The MunI (MfeI) restriction enzyme recognizes C^AATTG sites and cuts best at 37°C in G buffer (Isoschizomers: MfeI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | MunI (MfeI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nde I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest PstI
The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).
Sensitive to Heat Inactivation | No |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Pst I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 3' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).
Concentration | 10 U/μL |
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Thermo Scientific™ LguI (SapI) (5 U/μL)
The LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: BspQI, PciSI, SapI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | LguI (SapI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 5 U/μL |
Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).
Concentration | 10 U/μL |
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Thermo Scientific™ BshTI (AgeI) (10 U/μL)
Cut at A^CCGGT sites with BshTI (AgeI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AgeI, AsiGI, CspAI, PinAI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BshTI (AgeI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer O |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ ScaI (10 U/μL)
The ScaI restriction enzyme recognizes AGT^ACT sites and cuts best at 37°C in its own unique buffer (Isoschizomers: AssI, BmcAI, ZrmI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Sca I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Unique Buffer (10X Buffer ScaI) |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ HphI (10 U/μL)
The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | HphI |
Methylation Sensitivity | Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer B |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ BsuRI (HaeIII) (10 U/μL)
Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI).
Thermo Scientific™ Bpu1102I (BlpI) (10 U/μL)
Cut at GC^TNAGC sites with Bpu1102I (BlpI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Bpu1102I (BlpI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ Eco91I (BstEII) (10 U/μL)
The Eco91I (BstEII) restriction enzyme recognizes G^GTNACC sites and cuts best at 37°C in O buffer (Isoschizomers: BstEII, BstPI, EcoO65I, PspEI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Eco91I (BstEII) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer O |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ Cfr9I (XmaI) (10 U/μL)
The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Cfr9I (XmaI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | Unique Buffer (10X Buffer Cfr9I (XmaI)) |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ Eco147I (StuI) (10 U/μL)
The Eco147I (StuI) restriction enzyme recognizes AGG^CCT sites and cuts best at 37°C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Eco147I (StuI) |
Methylation Sensitivity | Dcm Methylation-Sensitive,Not Dam Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer B |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |