Dissociation Reagents

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ N-2 Supplement (100X)

N-2 Supplement (100X)

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Gibco™ B-27™ Supplement (50X), minus vitamin A

Suitable for use in most neuronal cell applications

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase Cell Dissociation Reagent

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ KnockOut™ Serum Replacement

KnockOut™ Serum Replacement

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ CTS™ (Cell Therapy Systems) N-2 Supplement

CTS™ (Cell Therapy Systems) N-2 Supplement

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Gibco™ 2-Mercaptoethanol (55mM)

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Cryopreserved Hepatocyte Recovery Medium (CHRM)

Cryopreserved Hepatocyte Recovery Medium (CHRM)

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures

Gibco™ HT Supplement (100X)

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ MEM Amino Acids Solution (50X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ Primary Hepatocyte Maintenance Supplements

Primary Hepatocyte Maintenance Supplements

Gibco™ Phytohemagglutinin, M form (PHA-M)

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

Gibco™ Poly-D-Lysine

Poly-D-Lysine is an extracellular matrix used to facilitate neural cell adhesion to tissue culture-treated plastic and glass surfaces. When used on Nunc plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

Corning™ BioCoat™ Poly-D-Lysine

Recommended for the culture of neurons, glial cells, and transfected cells

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