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Search results for "hitrap"

1 – 30 of 286 results
1

Cytiva HiTrap™ Desalting Prepacked Columns

For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.

Promotions are available
Particle Size >20μm (Dry), 15 to 88μm (wet)
Disposable No
Diameter (English) Inner Column 0.62 in.
Volume (Metric) Bed 5 mL
Storage Requirements 4 to 30°C, 20% Ethanol
Diameter (Metric) Inner Column 16 mm
2

Cytiva HiTrap™ Streptavidin HP Column

Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.

3

Cytiva HiTrap™ Protein A HP Prepacked Columns

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Binding Capacity 20mg IgG/mL medium
Storage Requirements 4 to 8°C, 20% Ethanol
For Use With (Application) Chromatography system|Manual
4

Cytiva HiTrap™ Capto™ S Column

Prepacked with a strong cation exchange modern resin for small scale protein purification

5

Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

6

Cytiva HiTrap Chelating Sepharose FF

Hitrap chelating sepharose FF

7

Cytiva HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Particle Size 34μm
Buffer 6M Guanidine Hydrochloride, 8M Urea
Ligand Type Iminodiacetic Acid
Height (Metric) 25mm
Flow Rate <20mL/min.
pH Range 3 to 13
Product Type HiTrap chelating HP column
Storage Requirements 4° to 30°C
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 3 bar
Height (English) 0.98 in.
8

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
pH Range 3 to 12
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Product Type HiTrap NHS activated HP column
Material Polypropylene
Storage Requirements 4° to 8°C
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
9

Cytiva HiTrap™ Butyl FF Columns

Prepacked columns for convenient and fast separations

Particle Size 45 to 165 µm
Ligand Type Butyl
Height (Metric) 25 mm
pH Range 3 to 13
Product Type HiTrap Butyl FF
Material Polypropylene
Storage Requirements 4°C to 30°C, 20% Ethanol
For Use With (Application) For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications
Support Type 4% Cross-linked Agarose
Media Type Butyl Sepharose 4 Fast Flow
Max. Pressure 5 bar [0.5 MPa] (70 psi)
Height (English) 0.984 in.
10

Cytiva HiTrap™ Con A 4B Columns

For separation and purification of glycoproteins, polysaccharides and glycolipids

Particle Size 45 to 165μm
Buffer Aqueous Buffer
Ligand Type Concanavalin A (13mg Con A/mL drained medium)
Height (Metric) 25mm
pH Range 4 to 9
Product Type HiTrap Con A Sepharose 4B column
Material Polypropylene
Storage Requirements 4° to 8°C
For Use With (Application) Convenient separation and purification of Glycoproteins, Polysaccharides and Glycolipids
Support Type 4% Agarose
Max. Pressure 3 bar
Height (English) 0.98 in.
11

Cytiva HiTrap™ Q Sepharose XL IEX Columns

Optimized for fast, convenient small-scale protein capture

Storage Requirements 4 to 30°C, 20% Ethanol
12

Cytiva HiTrap™ IEX HP Prepacked Columns

Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.

Storage Requirements 4 to 30°C, 20% Ethanol
13

Cytiva HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Height (Metric) 20mm
Flow Rate <500cm/hr.
pH Range 2 to 10
Product Type HiTrap Protein L column
Material Polypropylene
Storage Requirements 2° to 8°C
For Use With (Application) Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs
Support Type Highly Cross-linked Agarose
Max. Pressure 5 bar
Height (English) 0.78 in.
14

Cytiva HiTrap™ IEX Selection Kit

Use for fast and easy screening of a variety of different media. HiTrap™ IEX Selection Kits contain Sepharose™ Fast Flow and Sepharose XL in combination with seven different ion exchange ligands.

15

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Particle Size 34μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
pH Range 5 to 10
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Product Type HiTrap Heparin Sepharose HP prepacked column
Sample Type DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs
Material Polypropylene
Storage Requirements 4° to 30°C
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
16

Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns

Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.

Storage Requirements 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
17

Cytiva HiTrap™ DEAE Sepharose Fast Flow IEX Columns

Weak anion exchanger for small-scale protein purifications.  Cytiva Lifescience™ HiTrap™ DEAE Sepharose Fast Flow IEX Columns are convenient and rapid.

Storage Requirements 4 to 30°C, 20% Ethanol
18

Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns

Use for small-scale protein purifications.   Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.

Promotions are available
Storage Requirements 4 to 30°C, 20% Ethanol
19

Cytiva HiTrap™ MabSelect SuRe™ PCC Columns

Prepacked with MabSelect SuRe™ PCC Protein A Resin for screening and small-scale mAb purification in continuous bioprocessing using periodic counter-current chromatography (PCC)

Height (Metric) 25 mm
Matrix MabSelect SuRe™ PCC Protein A Resin
Description Flow Velocity: ≤ 600 cm/h
Product Type Chromatography Column
Capacity ~60 mg Human IgG/mL Resin
Storage Requirements Store at 2°C to 8°C, 20% Ethanol
For Use With (Application) Antibody Affinity Chromatography
Max. Pressure 5 bar (0.5 MPa)
20

Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

Promotions are available
Storage Buffer 20% Ethanol
Storage Requirements 4° to 30°C
For Use With (Application) Designed for use with a syringe, peristaltic pump, or chromatography system
21

Cytiva HiTrap™ MabSelect™ Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™ Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

Storage Buffer 20% Ethanol
Storage Requirements 4° to 30°C
22

Cytiva HiTrap™ Capto™ Q ImpRes IEX Columns

High-throughput medium for intermediate and polishing protein purification

Storage Requirements 4 to 30°C, in 20% Ethanol
23

Cytiva HiTrap™ Capto™ Q IEX Columns

Strong quaternary ammonium (Q) anion exchanger

Particle Size 90μm (d50V)
Matrix Highly cross-linked agarose with dextran surface extender
pH Range 2-12
Storage Requirements 4 to 30°C, 20% Ethanol
24

Cytiva HiTrap™ Capto™ HIC Selection Kit

Five Capto™ Hydrophobic Interaction Chromatography Resins in HiTrap™ Columns for resin selection and screening protein purification conditions

25

Cytiva HiTrap MabSelect PrismA Column

Protein A Column

Flow Rate <20mL/min
Bed Volume 5mL
Product Type Column
For Use With (Application) Antibody affinity chromatography
Pressure 5 bar (0.5 MPa, 70 psi)
26

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Particle Size 60 - 160 µm
Ligand Type Talon Superflow Cobalt-IMAC
Flow Rate 1 to 4 mL/min
Matrix 6% Cross-Linked Agarose
Binding Capacity Up to 20 mg Histidine-tagged Protein1
Storage Buffer 20% Ethanol
pH Range 3 to 12
Product Type HiTrap Talon crude prepacked Column
Material Polypropylene
Storage Requirements 4°C to 8°C
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Max. Pressure 3 bar
27

Cytiva HiTrap™ MabSelect™ SuRe Prepacked Columns

Use these columns in small-scale MAb purification in a continuous bioprocess. Cytiva Life Sciences™ HiTrap™ MabSelect™  SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes

Content And Storage Buffer 20% Ethanol
Description HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess resin for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).
Binding Capacity ≈35mg Human lgG/mL at 2.4 min. residence time
Storage Requirements 4° to 30°C
For Use With (Application) For fast purification of monoclonal antibodies from large sample volumes, affinity purification
28

Cytiva HiTrap™ IgM Purification HP Column

Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture

29

Cytiva HiTrap IXSelect

HiTrap IXSelect columns are prepacked with IXSelect resin for screening and small-scale protein purifications using affinity chromatography.

Storage Requirements 2 to 8 °C
30

Cytiva HiTrap Capto Butyl

Capto Phenyl (High sub) and Capto Butyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification

Height (Metric) 25 mm
Flow Rate < 600 cm/h
Storage Requirements 4 to 30 °C, 20% Ethanol
For Use With (Application) Hydrophobic Interaction Chromatography