Cell Function Reagents and Kits
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ Propidium Iodide Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Propidium Iodide binds to double-stranded DNA by intercalating between basepairs, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Propidium Iodide
Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450
A violet fluorescent dye that can be used to monitor individual cell divisions for up to 7 generations.
Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ DRAQ5™
Anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells.
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 670
A red fluorescent dye that can be used to monitor individual cell divisions for up to 6 generations.
Invitrogen™ eBioscience™ Calcein Blue AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Blue.
Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry PE
Contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. This kit is optimized to achieve brighter staining compared to traditional protocols.
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