DNA Extraction and Purification
DNA Extraction and Purification
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Filtered Search Results
Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.
Shipping Condition | Room Temperature |
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Content And Storage | • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
Final Product Type | Plasmid DNA |
Isolation Technology | Silica Spin Column |
Column Type | Spin Column |
Concentration | 20 mg/mL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation |
Test Time | 30 to 45 min. |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Target | Plasmid DNA |
Format | Spin Column |
Product Type | Quick Plasmid MiniPrep Kit |
Sample Type | Bacterial Culture |
Product Line | PureLink™ |
Cytiva TempliPhi™ 25 Amplification Sample Kit
For the preparation of circular DNA templates for cycle sequencing, cloning, and transformation
Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit
Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.
Format | Mini Spin Column |
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Efficiency | Typical recovery: 70–95% |
Size | 50 bp to ∼20 kbp |
Isolation Technology | Silica Membrane Technology |
Color | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
Purity | A260/A280: 1.8–1.9 |
Sample Volume | <400μL (PCR reaction mixture) <400mg (TAE / TBE agarose gel) |
Quantity | 1 Kit |
Yield | Up to ∼15μg DNA |
Test Time | 10 min/6 preps |
QIAGEN QIAcard FTA CloneSaver
For collection, stabilization, processing, transport and archiving of buccal nucleic acid samples. 96 sample areas per card.
Invitrogen™ PureLink™ Fast Low-Endotoxin Maxi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
Shipping Condition | Room Temperature |
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Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
Format | Kit |
Product Type | Plasmid Purification Kit |
Isolation Technology | Silica Column |
Scale | Maxi |
For Use With (Application) | Cloning, PCR |
Test Time | 30 min. |
Product Line | PureLink™ |
Prep Scale | Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg |
Cytiva MicroSpin™ S-400 HR Columns
Use for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides. Cytiva Life Sciences™ illustra™ MicroSpin™ S-400 HR Columns use spin-column chromatography for quick results.
Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | • Resuspension buffer, store at 4°C upon reciept • Lysis buffer, store at room temperature • Precipitation buffer, store at room temperature • Binding buffer, store at room temperature • Wash buffer 1, store at room temperature • Wash buffer 2 (concentrate), store at room temperature • Elution buffer, store at room temperature • Column assemblies, store at room temperature • Syringe filters, store at room temperature • Syringe plungers, store at room temperature • Collection tubes, store at room temperature |
Format | Kit |
Product Type | Plasmid Purification Kit |
Isolation Technology | Silica Column |
Scale | Midi |
For Use With (Application) | Cloning, PCR |
Test Time | 30 min. |
Product Line | PureLink™ |
Prep Scale | Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg |
Invitrogen™ PureLink™ Quick Gel Extraction Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages.
Shipping Condition | Room Temperature |
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Label or Dye | Ethidium Bromide, SYBR Safe |
Green Features | Less hazardous, sustainable packaging |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Test Time | 30 min. |
MP Biomedicals™ MERmaid™ SPIN Kit
MERmaid™ SPIN Kit for rapid purification of single or double-stranded DNA (10 to 200 bp) from gel material, nucleotides, salts, proteins, or other undesirable components of mixture.
MP Biomedicals™ MERmaid™ SPIN Kit
MERmaid™ SPIN Kit for rapid purification of single or double-stranded DNA (10 to 200 bp) from gel material, nucleotides, salts, proteins, or other undesirable components of mixture.
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MaxiPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Maxi |
Concentration | 400 ng/μL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
Test Time | 60 min. |
Product Line | GeneJET |
Prep Scale | > 200 μg (Large-Scale) Plasmid DNA |
Cytiva TempliPhi™ 2000 Reaction Kit
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications
MP Biomedicals™ Ready-Red™ Mixture DNA and RNA Extraction
Used to remove traces of phenol from the aqueous phase of organic nucleic acid extractions.
Content And Storage | Store at Room Temperature (15 to 30 °C) |
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Form | Solution |
Formulation | Chloroform:isoamyl alcohol 24:1 with red dye |
Purity | >99% |
DNase | Yes |
Molecular Formula | CHCl3, C5H12O |
RNase | Yes |
Thermo Scientific™ GeneJET Plasmid Midiprep Kit
Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MidiPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Midi |
Concentration | 400 ng/μL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
Test Time | 60 min. |
Product Line | GeneJET |
Prep Scale | 100-200 μg (Medium-Scale) Plasmid DNA |