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Life Science Buffers

Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

  • Temperature
  • Desired or target pH
  • Buffer toxicity (to the system)
  • Buffer interactions with other system components

 

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1 – 15 of 77 results
1

Gibco™ PBS, pH 7.2
GREENER_CHOICE

PBS, pH 7.2

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Dilution 1 X
Content And Storage 15°to 30°C
Additive No Sodium Pyruvate
Form Liquid
Product Type Phosphate Buffered Saline (PBS)
pH 7.2
Shelf Life 36 Month(s)
For Use With (Application) Mammalian Cell Culture
Osmolality 280 to 320 mOsm/kg
2

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

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Shipping Condition Room Temperature
Dilution 10 X
Form Liquid
Product Type Phosphate Buffered Saline (PBS)
pH 7.4
Product Line Ambion™
3

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Content And Storage Room temperature
Form Liquid
4

Gibco™ DPBS, calcium, magnesium
GREENER_CHOICE

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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Dilution 1 X
Additive No Sodium Pyruvate
Form Liquid
Product Type DPBS
Classification Animal Origin-Free
pH 7.0 to 7.2
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
For Use With (Application) Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents
Sterility Sterile-filtered
Phenol Red Indicator No Phenol Red
Solution Type Dulbecco's Phosphate Buffered Saline
Product Line Gibco™
5

Gibco™ DPBS, no calcium, no magnesium
GREENER_CHOICE

DPBS, no calcium, no magnesium

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Dilution 1 X
Content And Storage 15°to 30°C
Form Liquid
Product Type DPBS
6

PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

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7

TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

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Name Note 50X Solution
Concentration or Composition (by Analyte or Components) Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS 6850-28-8
Protease Protease free
Physical Form Liquid
Grade Electrophoresis
Synonym TAE
Purity Grade Notes DNase-, RNase- and Protease-Free
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
8

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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9

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

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Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
10

Invitrogen™ Novex™ Tris-Glycine Native Running Buffer (10X)

Use with NuPAGE Novex Tris-Acetate gels

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11

TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™

CAS: 10043-35-3 Synonym: TBE

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CAS 10043-35-3
Synonym TBE
12

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Color Colorless
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Filtered Through Filtered through a 5-micron filter.
ChemAlert Storage Symbol Gray
Name Note 1X Solution, pH 8.0
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention.

NFPA

Health:1
Flammability:0
Instability:0
Health Hazard 2 CAUTION!
pH 7.4 to 8.1
Synonym TE
Recommended Storage RT
13

Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)

Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.

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14

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

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Boiling Point 100°C
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris Buffered Saline
Grade Molecular Biology
DNase DNase free
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS 7732-18-5
Protease Protease free
pH 7.5
Synonym TBS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
15

Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)

Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels.

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