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Products

IBA Lifesciences™ Strep-Tactin-HRP Conjugate

Used for fast and direct detection of Strep-tag™II fusion proteins transferred to a blotting membrane or coated to a microplate. IBA Lifesciences™ Strep-Tactin-HRP Conjugate is suitable for chromogenic and chemiluminescence reactions. 500 UL STREP-TACTIN(R) HRP CONJUGATE 0.5ML STOREin 4C Fridge

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B.

IBA GmbH™ 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT. 25ML 10x Buffer BXT; Strep-TactinXT Elution Buffer

IBA GmbH™ Strep-Tactin™XT Superflow™ High Capacity

IBA GmbH™ Strep-Tactin™XT Superflow™ High Capacity Reliable is a one-step purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins with high affinity and unsurpassed purity. 50ml Strep-Tactin® XT Superflow® high capacity

IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension

Ideal for gravity flow purification of recombinant Strep-tag™ proteins. IBA Lifesciences™ Strep-Tactin™ Sepharose™ Suspension is conveniently formulated as a 50% Sepharose™ suspension. 20 ML Strep-Tactin(R) Sepharose 50 % suspension

IBA Solutions for Life Science™ Anhydrotetracycline

CAS: 1665-56-1 Molecular Formula: C22H22N2O7 Molecular Weight (g/mol): 426.43 InChI Key: JGRMIMMCZBOWCW-LCGGBVFXSA-N Synonym: anhydrotetracycline, 5a,6-anhydrotetracycline, 4s-anhydrotetracycline, cxcveerymjzmmm-docrcchosa-n, 4s,4as,12as-4-dimethylamino-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-1,4,4a,5,12,12a-hexahydrotetracene-2-carboxamide, anhydrotetracycline hydrochloride, 2-naphthacenecarboxamide, 1,4,4a,5,12,12a-hexahydro-4-dimethylamino-1,12-dioxo-6-methyl-3,10,11,12a-tetrahydroxy-, 4s-4-alpha,4a-alpha,12a-alpha, 2-naphthacenecarboxamide,4-dimethylamino-1,4,4a,5,12,12a-hexahydro-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-, 4s,4as,12as, anhydrotetracyclin, tdc PubChem CID: 54675758 50 MG ANHYDROTETRACYCLINE 50MG STORE IN -20CFreezer

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension

IBA GmbH™ Strep-Tactin™XT Superflow™ 50% Suspension can be used to pack your own columns for protein purification. Strep-Tactin XT Sepharose 50% suspension

IBA Solutions for Life Science™ MagStrep “type 3” XT Beads 5% Suspension

Have high binding capacity combined with very low non-specific protein binding 2ML MagStrep ''type3'' XT beads , 5% suspension

IBA Solutions For Life Science™ D-Desthiobiotin

Used for elution of Strep-tag™ II proteins from Strep-Tactin™ 1 GR D-DESTHIOBIOTIN 1G STORE IN 4C FRIDGE

IBA GmbH™ Gravity Flow Superflow™XT Column

IBA GmbH™ Gravity Flow Superflow™XT Column is for one-step purification of recombinant Strep-tag™II and Twin-Strep-tag™ fusion proteins with high affinity and unsurpassed purity. Gravity flow Strep-Tactin XT Superflow 10ml column

IBA GmbH™ Strep-Tactin™XT Superflow™ Cartridge

IBA GmbH™ Strep-Tactin™XT Superflow™ Cartridge is a reliable one-step purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins with high affinity and unsurpassed purity. Strep-Tactin Superflow XT 5ml cartridge (with 10-32 connection for HPLC and Äkta)

IBA Lifesciences™ Magnetic Separator

Aids fast purification of Strep-tag™ proteins. Magnetic Separator for 24 rxntubes, 1piece

IBA Solutions for Life Science™ Strep-tag™ Washing Buffer, 10X

100 ML Strep-tag(R) washing buffer (10x buffer W)

IBA Solutions for Life Science™ StrepMAB-Classic

For detection of N- or C-terminal or internal Strep-tag™ II specific monoclonal antibody 75 UG STREPMAB-CLASSIC HRP CONJUGATE (STREP-TAGII-HRP conjugate), 25-30 Western blots 75µg Store

IBA Solutions for Life Science™ D-Desthiobiotin Buffer E

25 ML D-DESTHIOBIOTIN, 25MM BUFFER SOLUTION (10XBuffer E) contains Tris pH 8, NaCl and EDTA 25mL

IBA GmbH Protein A Agarose 50% Suspension

Protein A Agarose for purification of monoclonal- and polyclonal antibodies using affinity chromatography Protein A Agarose 50% suspension; 10 ml

IBA Lifesciences™ Microtiter Plate

Strep-Tactin® coatedmicroplate, 1 plate, 1plate

IBA GmbH™ Strep-Tactin™XT Spin Column Kit

IBA GmbH™ Strep-Tactin™XT Spin Column Kit allows for rapid parallel purification of Strep-tag™ and Twin-Strep-tag™ fusion proteins. 1 KIT Strep-Tactin XT Spin Column Kit

IBA Solutions For Life Science Strep-Tactin™XT Superflow™ 5mL Cartridge

A high capacity cartridge that provides more protein compared to Strep-Tactin™ high cap for one-step purification of recombinant Strep-tag™II and Twin-Strep-tag™ fusion proteins with HPLC or FPLC. 5ML Strep-Tactin XT Superflow high capacity cartridge

IBA Solutions For Life Science™ Strep-Tactin™ Superflow™ High capacity, 50% Suspension

Used for purification of recombinant Strep-tag™ proteins under gravity flow and low pressure or FPLC 50 ML Strep-Tactin(R) Superflow(R) high capacity,50% suspension 50mL Store in 4C Fridge

IBA GmbH™ GFP-Twin-Strep-tag Control Protein

IBA GmbH™ GFP-Twin-Strep-tag Control Protein is for test purifications with Strep-Tactin™ and Strep-Tactin™ XT. GFP-Twin-Strep-tag control protein

IBA Solutions for Life Science™ D-Biotin

2 GR D-Biotin (not for Strep-tag applications) 2g

IBA Solutions for Life Science™ Strep-tag™ Regeneration Buffer, 10X

100 ML STREP-TAG(R) REGENERATION BUFFER WITH HABA(10x Buffer R) 100mL Store in 4C Fridge

IBA Solutions for Life Science™ Strep-Tactin™ Superflow™, 50% Suspension

Used for purification of recombinant Strep-tag™ proteins under gravity flow and low pressure or FPLC 1 LT Strep-Tactin(R) Superflow(R), 50 % suspension

IBA GmbH™ MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA GmbH™ MART-1 peptide ELAGIGILTV (HLA-A*0201) is a single peptide for stimulation of human MART 1-specific CD8+ T-cells. 1MG MART-1 peptide ELAGIGILTV (HLA-A*0201)

IBA Solutions For Life Science™ Strep-Tactin Sepharose Suspension

4 ML STREP-TACTIN(R) SEPHAROSE 50 %SUSPENSION 4MLStore in 4C Fridge

IBA Lifesciences™ 5mL Gravity Flow Column

5 ML GRAVITY FLOW STREP-TACTIN(R) SEPHAROSE COLUMN, 5 mL 00 Store in 4C Fridge

IBA GmbH CD3 Isolation Kit for FABian&trade, Mouse

For automated positive cell separation. The Isolation Kit for FABian&trade provides label-free target cells without the use of high affinity antibodies and magnetic beads CD3 Isolation Kit for FABian(R), mouse, 10 Kits

IBA GmbH CD4 Fab-TACS&trade Gravity Kit, Mouse

For manual positive cell separation. The Fab-TACS&trade Gravity Kit provides label-free target cells without the use of high affinity antibodies and magnetic beads CD4 Fab-TACS® Gravity Kit, mouse, 10 columns

IBA GmbH™ GFP-Strep-tag Control Protein

IBA GmbH™ GFP-Strep-tag Control Protein is for test purifications with Strep-Tactin™ and Strep-Tactin™ XT. GFP-Strep-tag control protein

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Featured Products

Immobilisation with Near Covalent Binding Affinity


Immobilization with near covalent binding affinity

Twin-Strep-tag™ Capture Kit for SPR Experiments

  • Indirect immobilisation of the ligand prevents the ligand to be inactivated or influenced by the immobilisation procedure
  • High affinity in low pM range enables long term measurements (T1/2 = 13 h)
  • Chip can be easily regenerated by an established procedure
  • Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag:Strep-Tactin XT interaction

Try our New High Affinity Strep-tag™ Technology


Try our new high affinity Strep-tag® system

Strep-Tactin XT 4Flow™ Starter Kit consists of:

  • Strep-Tactin XT 4Flow gravity flow column 
  • Buffer W (Strep-Tactin/Strep-Tactin XT Wash Buffer) 
  • Buffer BXT (Strep-Tactin XT Elution Buffer with Biotin) 
  • Buffer XT-R (Strep-Tactin XT Regeneration Buffer with Biotin MgCl2)
  • Strep-Tactin conjugated with HRP (for Western blot detection)
  • Short protocol

Exosome Isolation - Quick and Simple


Try our new high affinity Strep-tag® system

The Fab-TACS™ Exosome Isolation Technology offers:

  • Highly specific and reproducible isolations with minimal contamination in a source-independent manner
  • Quick and simple protocol, without magnetic beads or ultracentrifugation, no extra device needed
  • Label-free exosomes with intact biological functions due to reversible reagents
Cell
IBS's Cell Selection

Traceless Affinity Cell Selection


Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection

  • High purity, yield and viability
  • Label-free, non-activated cells
  • From whole blood, buffy coat, PBMCs or murine samples
  • Manual or automated cell separation

Magnetic Cell Selection


Magnetic Cell Selection

IBA's Streptamer™

  • Fully reversible
  • No stimulation of cells after cell separation
  • Optimal preservation of cell effector function 
  • Improved viability of cells 

Cell Stimulation & Expansion


Cell Stimulation & Expansion

CD3/CD28 Streptamers™

  • Completely reversible reagents
  • Termination of stimulation at any given point in time
  • Adjustable CD3 : CD28 ratio 
  • Bead-free

Peptides for T cell stimulation

  • Purity > 85 %
  • Endotoxin free

Fluorescent Cell Staining


Fluorescent Cell Staining

Detection of antigen-specific T cells with MHC I Streptamers™ and of receptor-specific T cells with Fab Streptamers™

  • Fully reversible
  • High specificity, sensitivity and reproducibility
  • No leftover of staining reagents on the cells after FACS sorting
  • Optimal preservation of cell effector function
  • Improved viability of cells
Protein
Protein

Cloning


Cloning & Transfection

More than 150 Twin/Strep-tag™ II expression vectors

  • Variety of Twin/Strep-tag II combined with His, FLAG or GST
  • Different N-/C-terminal orientations
  • Expression hosts: E. coli, mammalia, insect cells, yeast
  • Easy cloning via StarGate vectors

Mammalian Expression


Cloning & Transfection

The MEXi mammalian expression system

Optimised for efficient protein expression and Strep-tag purification in mammalian cells.

  • Perfectly matched HEK293E cell line and cell culture media
  • Transient expression for time and cost efficient protein production
  • Multiple expression vectors from IBA with different features

Protein Purification


Protein Purification

Strep-tag™ technology from the original manufacturer

  • High affinity in low pM range
  • Rapid one-step purification with unparalleled protein purity (> 95 %)
  • Mild and specific elution
  • Tolerates broad range of buffer conditions
  • Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other

Detection


Detection

For Western blot analysis, immunofluorescence, FACS and ELISA

Two different detection systems are provided:

  • Strep-Tactin and Strep-Tactin XT conjugate for Twin/Strep-tag II proteins
  • Monoclonal antibodies against Twin/Strep-tag II

Immobilisation


Immobilisation & Immunoprecipitation

Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI

  • High affinity in low pM range enables measurements over a long time
  • Minimal non-specific binding 
  • Minimal coefficients of variation (cv)
  • Chip for SPR experiments can be easily regenerated
Exosomes

Traceless Affinity Exosome Isolation


Backbone Modifications

Quick and simple isolation of unlabelled exosomes using Fab-TACS™ technology

The Fab-TACS technology for exosome isolation offers

  • Highly specific and reproducible isolations
  • Minimal contamination by other EVs
  • Label-free exosomes with intact biological functions
  • Source-independent application
  • Reversible capture and release of exosomes
  • A quick and simple protocol, without magnetic beads or ultracentrifugation
Resources Strep-Tactin XT 4Flow Sample Request
IBA_about_us

About IBA GmbH

Solutions For Life Sciences

IBA Lifesciences provides innovative technologies for life science applications. The product portfolio includes tools for cell selection and expansion as well as protein production and assays.