Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Brand:  Applied Biosystems™ 4312704

Product Code. 10666175

Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.

Description

Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
• Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

• Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
• Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.
  
  Store at -20°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  
  
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Label or Dye: SYBR Green
• Product Line: SYBR™
• Quantity: 10 x 5 mL
• For Use With (Application): Gene Expression
• No. of Reactions: 2000 Reactions
• Volume: 10 x 5 mL

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Sufficient For: 2000 Reactions of 50 μL
• Concentration: 2X
• Sample Type: DNA (Genomic), cDNA

Safety and Handling

Product Identifier

• Green PCR Master Mix

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362-Take off contaminated clothing.

Supplemental information

• MIXTURE LIST-Contains : Tris (hydroxymethyl)aminomethane, Sodium azide

For Research Use Only. Not for use in diagnostic procedures.